The First Presbyterian Church building has become a beloved historical landmark for the community, as well as a Church home for the members of the Church, for it is the oldest public building in Tallahassee.

In 1824, Governor Duval, by proclamation located the capital of the Territory of Florida at the site which has become the City of Tallahassee. When the Legislative Council convened in the fall of 1826, a Presbyterian Minister, Reverend Henry White, was appointed Chaplin and given permission to hold preaching services each Sabbath while the Assembly was in session in the partially constructed wing of the capitol building. The structure was torn down in 1839 and the present capitol was begun.

From 1827 through 1831 the records of the Board of National Missions of the Presbyterian Church show home missionaries assigned to this field, and a Sunday School organized. Late in 1832 the Reverend Joseph Stiles, Horace Pratt and Nathaniel Pratt, a committee from the Georgia Presbytery, held a "protracted meeting" and on November 4, 1832 organized a Presbyterian Church composed of two Ruling Elders and sixteen members. It was placed under the temporary care of Hopewell Presbytery. On February 16, 1833 the Church was incorporated by act of the Territorial Council. In June 1835 the congregation asked for bids to erect a brick building 68 ft. x 48 ft. The building was finished three years later and dedicated to the Lord on Sunday, May 13, 1838. Financing of the Church was by the sale of pews, the lowest bid being $125.00, the total pew sales $12,500. The final building costs totaled $13,370.60. The pews of mahogany, with doors, were later replaced by the present pews.

The timber used in the building was of heart pine. Some idea of its quality can be gained from the table top in the entry of the Church. This top was the original entrance door sill, hollowed out by the passage of many feet through the years, and now squared up and preserved as a historical piece in the form of a table.

Pillars supporting the galleries were hand-hewed and show the adze marks if one looks closely. The north gallery was reserved by the trustees' deed for slaves who attended the services. They were admitted to membership.